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The Scientist and her Cats

The musings of a scientist (in training) as she navigates life.

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Making media 👩‍🍳 . I’ll be growing up a lot of cells for experiments in the next few weeks so I had to make up a fresh bottle of media. For my cancer cells we use RPMI (from @gibcocellculture ) and add 10% FCS or NCS (depends on the cells), HEPES buffer and then a selection of antibiotics (penicillin, gentamicin and 2-ME). In our lab we call it R10 media (is this a wide thing or a name our lab made up? 😅) Wish me luck for all the experiments this media is going to be put to work in! . #science #scientist #research #researcher #cellculture #cancerresearch #phdstudent #phdlife #nunccellculture

Was going to try to do heaps of work today but decided to only do what was needed so I could head home and binge watch my newest guilty pleasure show 😅 Apart from checking mice I had to freeze the surgery samples I collected yesterday. They were kept in RNAlater overnight and now they get to stay in the -80C until I decide which samples I will get sequenced in a couple months time. . #scientist #researcher #womeninstem #science #research #medicalresearch #cancerresearch #phdlife #phd #timelapse

Cats 🥰. . I am grant writing at the moment, so there are not many opportunities to take pictures in the lab...so I may as well use this time to showcase my cats! I have two cats, Bucky (who I’m holding in the picture) and Schrodinger. They are honestly just the best and love (or probably are indifferent to) being picked up, hugged and annoyed all the time. Bucky also gets picked up and I pretend he is a plane and fly him around the room 😅 He is a pretty big cat so that’s why I’m struggling to hold him and take a photo at the same time. . #cats #catsofinstagram #scientist #researcher #phdlife #phdstudent

Splitting Cells 🧪 . . Lots of my time is spent in one of our tissue culture labs. After 3.5 years I’ve gotten pretty efficient at splitting my cells and I can do it on autopilot. This time lapse covers the 15-20 minutes it takes me to split my cell, from start to finish. . The hood isn’t that bright too, it is just the contrast between the hood light, the darker room (it was night time) and the exposure of the video 😅 . #medicalresearch #scientist #science #research #researcher #phd #phdlife #phdstudent #cancerresearch #tissueculture #womeninstem

Cell culture 🧫 . Cells need a lot of tender love and care...so that means I’m often in the tissue culture hood on the weekend. Today I had to make up some new media for my two cancer cell lines I am currently working with. I’ll be in tomorrow (Sunday) as well to check on my cells and get them ready for the upcoming week of experiments! . #science #scientist #medicalresearch #research #researcher #cancerresearch #cellculture #nunccellculture #phdlife #phdstudent #phdstudentlife

Teaching 👩🏼‍🏫. . Today I spent most of my day teaching some year 12 human biology students! Todays class was all about PCR and gel electrophoresis, looking for a mutation in the BRAF gene in melanoma samples. I adore teaching and love being in the lab with excited and enthusiastic high school students. Tomorrow I get to do another class, this time about stem cells and cell culture. Anyone else teach? Teaching has improved my public speaking and ability to explain science so much! And I just love talking about science 😅 . #medicalresearch #science #research #phdstudent #cancerresearch #teaching #teacher #sciencecommunication #scicomm #researcher #scientist

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